Total erk antibody
WebAug 1, 2003 · To determine whether primary Reed-Sternberg cells also aberrantly express p-ERK1/2, we studied sections of HD lymph nodes by immunohistochemical techniques using antibodies to total ERK and phospho-specific antibody to p-ERK1/2. Sections from normal tonsils (n = 4) were stained for total ERK and p-ERK and were used as controls. WebOct 22, 2024 · (C) Levels of p-Erk, Erk and bax in the control group, vehicle group, anti-TNF-α antibody group and anti-TNF-α antibody+PMA group were detected by Western blotting. ( …
Total erk antibody
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Web19 rows · Erk Antibody Comparison Table. Phospho-Specific Protein p44/42 MAPK (Erk 1/2) Antibody Comparison Table. No. Antibody Reactivity WB IP IHC Flow IF; No. Antibody: … WebThe Erk1/2 antibody confirms silencing of Erk1 expression, and GCK Antibody is used to control for loading and specificity of p44 MAPK (Erk1) siRNA. Western blot analysis of … A Protein Modification Resource. PhosphoSitePlus ® is an online systems … Here are answers to the most frequently asked questions. If for any reason you … Primary Antibodies (7986) Secondary Antibodies (71) Antibody Conjugates … Terms and conditions for product sale and use - Cell Signaling Technology Quickly identify your next research target using the interactive pathways, diagrams, … Changes. Information about the cookies used by us may be updated from time to … Doing good science is important. So is just doing good. Cell Signaling Technology … The majority of our products are initially tested and approved by western blotting. …
Web9/28/2024 Good Total ERK Antibody YONG M This antibody was used to detect total ERK expression in prostate cell line PNT2, PC3 and ... Read Review (1) Write a Review. Santa … WebIn another experiment, cells were pretreated with 2 mg/mL integrin β1-neutralising antibody, total protein was extracted using lysis buffer to analyze FAK, p-FAK, Erk, pErk, Elk1, ... Erk, and Elk1 relative to total FAK, Erk, and Elk1 were gradually increased from 0.5 h to 12 h after IGFBP2 treatment, and then decreased from 12 h to 48 h ...
WebOct 22, 2024 · (C) Levels of p-Erk, Erk and bax in the control group, vehicle group, anti-TNF-α antibody group and anti-TNF-α antibody+PMA group were detected by Western blotting. ( D ) Quantitative analyses showing the levels of p-Erk relative to total Erk, and bax relative to β-actin normalized to sham-operated control group (n=5/group, ** p <0.01 versus sham … WebSanta Cruz Biotechnology, Inc. offers a broad range of ERK antibodies. Select ERK antibodies from monoclonal antibodies listed below. View detailed ERK antibody …
WebSpecificity / Sensitivity. p44/42 MAP Kinase (L34F12) Mouse mAb detects endogenous levels of total p44/42 MAP kinase (Erk1/Erk2) protein. In some systems this antibody may …
WebSep 28, 2024 · This antibody was used to detect total ERK expression in prostate cell line PNT2, PC3 and DU145. The data showed ERK can be detected in all of three cell lines. … dramatist\u0027s inWebMay 1, 2009 · For total ERK, membranes were stripped for 30 min in a solution of 62.5 mM Tris HCl, 2% SDS, and 100 mM β-mercaptoethanol, and reprobed using a mouse monoclonal antibody to total ERK (1:1000, #4696, Cell Signaling Technology, Beverly, MA) followed by an Alexa 680 conjugated goat anti-mouse secondary antibody (1 : 10 000, Molecular Probes). dramatist\u0027s imWebSanta Cruz Biotechnology, Inc. offers a broad range of ERK antibodies. Select ERK antibodies from monoclonal antibodies listed below. View detailed ERK antibody specifications by linking to the specific product blocks. Select appropriate ERK antibodies for your research by isotype, epitope, applications and species reactivity. dramatist\u0027s ioWebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699) Research with confidence – consistent and … radson javaWebJul 27, 2000 · a, Following stimulation with crosslinked anti-CD3 (KT-3) and anti-CD4 (GK1.5) antibodies, we analysed induction of ERK, JNK and p38 phosphorylation by sequential … radson optimoWebJun 25, 2012 · The total and phosphorylated protein levels of Runx2 were detected using Western blotting. As shown in Figure 4, there were obvious differences in the phosphorylation levels of ERK and p38 between the MT01-treatment and control groups; p-ERK and p-p38 were significantly inhibited in the presence of U0126 and SB203580, which … radson optimo 800Web7. Dilute the primary antibody in TBST to the recommended dilution. We recommend incubating in a sealed bag, hybridization tube or 50 ml Falcon tubes (~2.5 ml primary antibody/blot). Incubate overnight at 4°C with agitation. 8. Rinse the blot in TBST three to four times for 5 min each at room temperature. 9. dramatist\u0027s ii