Webb11 apr. 2024 · The decrease of antibody efficacy to mutated SARS-CoV-2 spike RBD explains the breakthrough infections and reinfections by Omicron variants. Here, we analyzed broadly neutralizing antibodies ... Webb20 apr. 2015 · About 90 thermostabilizing mutations were identified in GPCRs by alanine scanning so far, however, only two of these mutations were predicted to be transferable …
Computational Thermostabilization of an Enzyme Science
WebbStreptomyces mobaraenesis transglutaminase can catalyze the cross-linking of proteins, which has been widely used in food processing. In this study, we rationally modified flexible regions to further improve the thermostability of FRAPD-TGm2 (S2P-S23V-Y24N-E28T-S199A-A265P-A287P-K294L), a stable mutant of the transglutaminase constructed in … Webb5 nov. 2015 · The data indicate that receptors are stabilized through a combination of factors, including an increase in receptor rigidity, a decrease in collective motion, reduced stress at specific residues, and the presence of ordered water molecules. Predicting thermostabilizing mutations computationally represents a major challenge for the field. lindenhurst ny obituary
Enhanced Thermostability and Catalytic Activity of
Webb13 apr. 2024 · Next, we mutated several residues in IRR that correspond to those in sites-1 and -2 of IR for insulin binding 26,28, and examined their effects on IRR activation at pH 7.4, 8.0 and 9.0 in the ... Webb1 jan. 2016 · Predicting Thermostable Mutations. Computational prediction of thermostabilizing mutations is a challenging process because the thermostability could … Webb24 dec. 2024 · The β-glycoside hydrolases (LXYL-P1−1 and LXYL-P1−2) from Lentinula edodes (strain M95.33) can specifically hydrolyze 7-β-xylosyl-10-deacetyltaxol (XDT) to form 10-deacetyltaxol for the semi-synthesis of Taxol. Our previous study showed that both the I368T mutation in LXYL-P1−1 and the T368E mutation in LXYL-P1−2 could increase … hothead kirby