Promoter deletion analysis
Promoter bashing is a technique used in molecular biology to identify how certain regions of a DNA strand, commonly promoters, affect the transcription of downstream genes. Under normal circumstances, proteins bind to the promoter and activate or repress transcription. In a promoter bashing assay, specific point mutations or deletions are made in specific regions of the promoter and the tran… WebSep 1, 2002 · Promoter deletion analysis revealed that the region from –223 to +111 bp is sufficient to direct trichome‐specific expression, but not strong gland expression. Examination of the literature suggests that this is the first characterized trichome‐specific‐promoter shown to function at all stages of plant development.
Promoter deletion analysis
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WebPromoter deletion analysis was conducted to identify the cis -acting elements of the promoter responsible for controlling root-specific expression. The GUS reporter gene was fused with various deletion fragments of the OsAER1 promoter and the resulting constructs were transformed in rice plants to generate transgenic plants. WebA detailed deletion analysis of the upstream sequences of the AP2 gene was done to identify the promoter. GUS assay of transgenic A. thaliana plants carrying various AP2 upstream fragments fused to the uidA gene showed that ~200-bp 5' UTR sequences are capable of driving gene expression at low levels in vegetative tissues whereas inclusion of …
WebFeb 5, 2013 · To understand its regulatory mechanism, here we define a novel JA-responsive cis -element (JARE), G (C)TCCTGA, in the AtJMT and BcNTR1 promoters, by promoter deletion analysis and Yeast 1-Hybrid (Y1H) assays; the JARE is distinct from other JA-responsive cis -elements previously reported. WebJan 27, 2024 · Promoter Deletion Studies DNA fragments corresponding to the upstream sequences of the AP2 gene were PCR amplified using specific primers (Table 1) and placed upstream of the uidA gene in the pORE-R2 vector [ 20 ]. These fragments were named as D1 (−3306/+225), D2 (−627/+225), D3 (−346/+225), D4 (−191/+225), D5 (−83/+225), and D6 …
WebSep 6, 2016 · Promoter 5’-deletion analysis demonstrated that truncated GhAO1 promoters with serial 5’-end deletions had different GUS activities. A 360-bp fragment was sufficient to activate GUS expression. WebA, MPG1 promoter 5 deletions used in this study in relation to the full length MPG1 promoter. The length of the promoter in each truncation is indicated (arrows). Also shown …
WebJ. Bacteriol.€1995, 177(15):4508. S A Fletcher and L N Csonka € Salmonella typhimurium. transcriptional silencer of the proU operon of Fine-structure deletion analysis of the
WebFeb 25, 2013 · Promoter deletion analysis is one of the primary and most widely used techniques employed to determine whether they are cis-acting elements or specific transcription factor binding sites within a... how many bonobo are leftWebPromoter deletion analysis revealed that DNA elements residing in the 5′ UTR are required for the salt induced expression of AtSOT12. Cytosine methylation in the promoter was low and salt stress slightly increased the DNA methylation level, suggesting that DNA methylation may not contribute to AtSOT12 gene repression. how many bonobos stores are thereWebApr 20, 2024 · Promoter deletion analysis of the human SLC25A13 gene in region of c.-221 to c. -1. A series of 3 truncated fragments were cloned into the pGL3 basic-LUC vector and … high prf radarWebSep 14, 2024 · Liquid biopsy provides real-time monitoring of tumor evolution and response to therapy through analysis of circulating tumor cells (CTCs) and plasma-circulating tumor DNA (ctDNA). USP44 is a critical gene which plays an important role in cell proliferation; however, its accurate role in other cellular networks is under research. USP44 promoter … high priaie sportsman onyoutnbeWebApr 21, 2016 · The deletion analysis showed that fragments D1–D8 responded well to NaCl and PEG stresses, whereas fragment D9 and CaMV 35S did not. The D8 segment (219 bp; -219 to -1 bp) exhibited the highest promoter activity of all tissues, with the exception of petals among the D1–D9 transgenic tobacco, which corresponds to about 10% and 25% … how many book does daring do haveWebDeletion analysis of Pichia pastoris alcohol dehydrogenase 2 (ADH2) promoter and development of synthetic promoters Biotechnol J. 2024 Feb;17 (2):e2100332. doi: 10.1002/biot.202400332. Epub 2024 Dec 17. Authors Fidan Erden-Karaoğlan 1 2 , Mert Karaoğlan 1 2 , Gürkan Yılmaz 2 , Semiramis Yılmaz 3 , Mehmet İnan 2 3 Affiliations high prfile roho cushion hcpcs codeWebpromoter by cloning the Lin28B proximal promoter for bioinformatics analysis. Next, a series of deletion vectors were constructed based on the bioinformatics analysis results for dual-fluorescein activity detection. The transcriptional regulation mechanism of the Lin28B promoter region was an- how many book pages is 12000 words