SpletExpression-ready Mouse CXCR3 cDNA ORF clone (MG50842-NF) with enhanced promotor in expression vector (pCMV3-SP-N-FLAG) is confirmed by full-length sequence and validated in expression capability for gene expression studies or other applications. Quote for bulk production. SpletpCMV-N-His是碧云天自行研发的用于在哺乳动物细胞中表达N端和His tag(His标签)融合的目的蛋白的表达质粒。含有CMV启动子可以高效启动目的蛋白在细胞中的表达;在多克隆位点的5'端含有一个可以编码His标签的序列,因此可以表达出含有His标签的融合蛋白,可以方便地使用抗His的抗体来识
pCMV-N-His(pCMV-N-His质粒)(D2737-1μg) - beyotime.com
SpletExpression Vector pCMV3-N-His Information Description His Tag Information A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine … Splet31. maj 2024 · fection. Cells were then transfected with pCMV3-SP-N-His (pCMV3, control group) (Sino Biological, Beijing, China) or pCMV3-MMP9 (MMP9 overexpres-sion group) (Sino Biological, Beijing, China) at a final concentration of 0.8lg/ml by Lipofectamine 2000 (Thermo, USA). After 48 h, cells were stimulated with different concentrations of P ... bruto neto kalkulaator 2022
pCMV3-SP-N-Flag Vector Sino Biological
SpletEnhanced CMV promoter Vector pCMV3-SP-N-His Restriction Sites KpnI + XbaI (6kb + 0.39kb) Tag Sequence His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC Sequencing Primers T7 ( 5' TAATACGACTCACTATAGGG 3' ) BGH ( 5' TAGAAGGCACAGTCGAGG 3' ) Quality Control The plasmid is confirmed by full-length … SpletVector : pCMV3-SP-N-His Shipping carrier : Each tube contains approximately 10 μg of lyophilized plasmid. Storage : The lyophilized plasmid can be stored at ambient temperature for three months. Quality control : The plasmid is confirmed by full-length sequencing with primers in the sequencing primer list. Sequencing primer list : but SpletpCMV3-SP-N-His Mammalian expression vector: Protein expression will be driven by the CMV promoter with N terminally fused His tag. The vector is specially suitable for … Here you can find our complete kits for gene knockout via CRISPR.All products … Expression vectors, like cloning vectors, are used for horizontal gene … A plasmid is a small, circular, double-stranded DNA molecule within a cell that … antibodies-online Inc. Jones Boulevard 321 Limerick, PA 19464 United States Phone … bruto nationaal inkomen kenia 2021