Web22 feb. 2024 · For the FM4-64 stained embryos the gravid worms were cut in egg buffer containing 2 μg/ml FM4-64 and immediately imaged. For the analysis of intestinal ER marker SEL-1(1–79)::mCherry::HDEL, the mCherry signals were detected at 500–520 nm and images taken in the GFP channel were used to identify the autofluorescence of gut … Web28 okt. 2024 · All images are single confocal sections. Bars, 25 µm. (e) Co-localisation of REM1.2-YN, VPg-YC and mCherry-HDEL on ER in N. benthamiana leaves at 2 dpi. The colour of interaction of REM1.2-YN and VPg-YC was digitally changed to green fluorescence to optimise merging with red fluorescent signal of mCherry-HDEL. All images are single …
GFP-TIP2;1 accumulates in the Endoplasmic Reticulum in C834 …
Web18 mrt. 2024 · To generate the promoter-reporter constructs, the CaMV35S promoter and EYFP CDS were replaced by sequences encoding the signal peptide of AtWAK2 (Sp … Web17 aug. 2024 · Plasmid pHIPX7 BiP N30 –mCherry–HDEL was constructed as follows: first, a PCR fragment containing BiP was obtained with primers KN18 and KN19 using WT … tpv world
A conserved gene regulatory network controls root epidermal cell ...
Web20 aug. 2024 · The persistent areas of mCherry-HDEL and SYT1-mGFP are pseudocolored green and magenta, respectively. The bottom right is a merged image of the first image of mCherry-HDEL (i) and the persistent area of SYT1-mGFP (ii). EPCS with SYT1-mGFP found in the edges of the sheet became an immobile tubule (arrowheads). Web19 dec. 2024 · S. cerevisiae BY4742 was washed twice after 2 h incubation with K28-mCherry HDEL and analyzed by confocal laser scanning microscopy. b Same cells analysed by structured illumination microscopy (SIM). The hypersensitive yeast strain 192.2d was treated with K28-mCherry HDEL as described in a and subjected to SIM. thermostat location kenmore dryer 400