Halo tag vector
WebHaloTag® Custom Vectors and Ligands HaloTag® technology is a comprehensive platform for functional protein analysis based on the formation of a covalent bond between a … WebCombined with OmicsLink™ expression-ready clones GeneCopoeia offers HaloTag® technology in a variety of mammalian and lentiviral expression vectors with the CMV promoter and neomycin selection marker. A …
Halo tag vector
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WebDescription All vectors are compatible with Promega Flexi Vector System, directional cloning method for protein-coding sequences Vectors designated “pFC” are configured to append HaloTag 7 to carboxy-terminus of … WebThe pHTC HaloTag® CMV-neo Vector is a simple multiple cloning site (MCS) plasmid for traditional cloning. It is designed for use with other HaloTag® C-terminal vectors for expression of C-terminal-tagged HaloTag® fusion proteins in mammalian cells.
WebDec 17, 2024 · If HiBiT is used, it should always be appended to the extreme terminus of the N- or C-HaloTag insertion by including its sequence in the dsDNA CRISPR donor vector (Fig. 1). This procedure uses a purified Cas9 protein, a site-specific guide RNA/trans-activating CRISPR RNA (guide RNA/tracrRNA) duplex, and a dsDNA donor plasmid … WebThank you ^v^. Having much fun with vector layers lately xD. I also made an improved version that is way better (this here was kinda rushed). It should be visible on my profile if you're interested :)
WebHaloTag®技术 ; shRNA相关技术 ... House keeping gene promoter positive control in single-reporter vector: 875 . WebThe HaloTag®-CRBN Fusion Vector contains the coding region for an N-terminal HaloTag® fusion to the E3 ligase component Cereblon (CRBN). The vector is designed for transient or stable fusion protein expression in mammalian cells and use in a NanoBRET™ assay format to measure the ternary complex formation between a target protein, a …
WebHaloTag® Fusion (N-Terminal) Mammalian Expression Vectors Inducible T7-Driven Flexi® Vectors for E. coli Expression このベクターは哺乳類細胞においてC末端にHaloTag®を …
Web• Labelling of cells with particular HaloTag ligand • Formation of a vector that encodes the fusion of HaloTag protein to a protein or protein-domain [6]. When enzyme and particular ligand come in contact, formation of an alkyl-enzymeintermediatetakes placewhennucleophilic displacement of terminal ms4sc-ap ソケットWebpFN28A HaloTag™ CMV-neo Flexi™ vector: Each for $320.50 Add to cart PRG8451 View Documents View Product Certificates. Promega G8451 0.1 μg/μL: 10mM Tris HCl, 1mM … ms4sa-dl カタログWebThe constructed vector was transformed into Rosetta 2 (DE3)-competent cells (Sigma-Aldrich). ... In another study using mouse embryonic stem cells (mESCs) expressing endogenous RAD21 with a Halo-tag, 109,400 RAD21 molecules per cell were estimated . Although it is difficult to make a strict comparison between the amounts of cohesin in … ms4sy-ap ソケットWebThe HaloTag® fusion vectors are designed to be used as the fluorescent acceptor in NanoBRET™ assays that monitor interactions of a specific target protein along the UPS pathway. NanoBRET™ technology is a … ms4u リレーWebThis system allows different fluorescent colours to be used to visualize the localization of the non-fluorescent HaloTag protein within living cells. A vector was constructed which expresses the HaloTag protein under the control of … ms4sm ソケットWebThe pFC14A and pFC14K HaloTag® CMV Flexi® Vector are configured to append HaloTag® to the carboxyterminus of the protein fusion partner and provide constitutive … ms4sm-ce ソケットWebpMH-Halo tag (Search Vector Database) Backbone size (bp) 7412 Vector type Mammalian Expression Selectable markers Neomycin (select with G418) Tag / Fusion Protein Halo tag (N terminal on backbone) Growth in … ms4sy-ap カタログ