WebDec 5, 2024 · Representative cytometric histograms of Cyto-ID staining in lymphocytes (a1 and b1), monocytes (a2 and b2), and granulocytes (a3 and b3) from one patient with rheumatoid arthritis (RA) and one healthy control subject (HC).Comparisons of autophagosome levels reflected by Cyto-ID staining mean fluorescence intensity in … WebCyto-ID assay is based on the usage of a specific dye that selectively stains autophagic compartments and therefore allows determination of autophagic flux as accumulation of …
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WebOct 20, 2016 · 3.3 Cyto-ID Staining 1. Resuspend cell pellets in 0.25 mL phenol red-free RPMI medium containing 5 % FBS. 2. All the following steps are performed in the dark ( see Note 4 ). 3. Add 0.25 mL diluted Cyto-ID ® working solution to each tube and mix well ( see Note 7 ). 4. Incubate in a cell incubator for 30 min at 37 °C and 5 % CO 2. 5. WebCyto-ID assay is based on the usage of a specific dye that selectively stains autophagic compartments and therefore allows determination of autophagic flux as accumulation of stained compartments in basic or activated conditions [rapamycin (15 µmol/L-), PP242 (1-5 µmol/L) or Hanks’ Balanced Salt Solution containing 6 mmol/L glucose (starvation … the lu blu restrarant in high point nc
Flow Cytometric Analysis of Autophagic Activity with Cyto-ID Staining ...
WebJul 19, 2024 · Cyto-ID staining measures autophagic flux in live cells by selectively labeling autophagic vacuoles that accumulate during autophagy 37, 38, and thus is used to quantify active autophagy. WebJun 26, 2024 · LysoTracker + (top) and CYTO-ID + (bottom) vesicles are shown in the background of MAP2 neuronal staining (n = 3 biological replicates). Scale bars are 25 μm. Scale bars are 25 μm. Source data ... WebOct 9, 2024 · Results: Firstly, the titanium implant micropits-nanotubular surface with ~30 nm diameters (MNT) can induce the M2 polarization of RAW264.7 spontaneously, as indicated by the spindle-like cell morphological alteration and specific molecular marker arginase-1 (Arg1) expression. tic tac toe snowman